Translocation of Borrelia burgdorferi surface lipoprotein OspA through the outer membrane requires an unfolded conformation and can initiate at the C-terminus
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Expression and Purification of Recombinant Outer Surface Protein D of Borrelia burgdorferi
To carry out the immunological experiments on the serum of Multiple Sclerosis (MS) patients, based on a correlation between Borrelia burgdorferi infection and contracting MS autoimmune disease the outer surface protein D (OspD) of the bacterium was expressed and purified. A clone containing the OspD gene in pET11a expression vector under the control of T7 promoter was transformed to the bacteri...
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INTRODUCTION Lyme borreliosis, caused by Borrelia burgdorferi sensu stricto in the United States and by several Borrelia species in Europe and Asia, has a great impact on the health of the global population. There are human vaccines available, such as the outer surface protein A (OspA) vaccine, but still more evidence is needed to verify its function. We investigated the safety, immunogenicity,...
متن کاملIdentification of Two Epitopes on the Outer Surface Protein A of the Lyme Disease Spirochete Borrelia burgdorferi
A murine IgM monoclonal antibody (MA-2C6) with κ-light chains directed against an antigenic determinant of outer surface protein A (OspA) of the Lyme disease spirochete, Borreliaburgdorferi, is produced. This antibody could bind specifically to OspA antigen of several isolates of B. burgdorferi, but not to the non-Lyme disease bacteria such as T. pallidum and B. hermsii. Antibody MA-2C6 was pur...
متن کاملRelationship between immunity to Borrelia burgdorferi outer-surface protein A (OspA) and Lyme arthritis.
Antibiotic-refractory Lyme arthritis may result from Borrelia burgdorferi-induced autoimmunity in affected joints. Such patients usually have certain HLA-DRB1 molecules that bind an epitope of B. burgdorferi outer-surface protein A (OspA₁₆₃₋₁₇₅), and cellular and humoral immune responses to OspA are greater in patients with antibiotic-refractory arthritis than in those with antibiotic-responsiv...
متن کاملBorrelia Outer Membrane Surface Proteins and Transmission Through the Tick
Pathogens of the bacterial genus Borrelia differ from fellow spirochetes, Treponema and Leptospira , in their use of arthropod vectors for transmission between warm-blooded hosts. The agents of relapsing fever, Borrelia recurrentis and Borrelia hermsii , are transmitted by lice and fast-feeding soft ticks, respectively , whereas the Lyme disease spirochete, Borrelia burgdorferi (Bb), is transmi...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2010
ISSN: 0950-382X,1365-2958
DOI: 10.1111/j.1365-2958.2010.07172.x